[Evaluation of Ocimum basilicum effects on uterus apoptosis in rats exposures in electromagnetic fields]

In the industrial world, almost everyone is unavoidably exposed to ambient electromagnetic field [EMF] generated from various technical and household appliances. Controversy exists about the effects of EMF on various tissues of the living bodies. Uterus play an important role in reproductive and the effects of EMF on its tissue is worthy of investigation. In order to examine this, 40 rat were selected and kept for one weeks in quarantine and 20 rats subdivided to two groups were exposed to 50 Hz [non-ionizing radiation] for 6 weeks, one of this exposed group received 0.7 g/kg Ocimum basilicum, other experimental group just received 0.7 g/kg Ocimum basilicum, The materials were processed and observed apoptosis under a light microscope. In the experimental rats apoptotic cells showed significantly increased in emf groups, but in exposed group that received Ocimum basilicum, apoptotic cells were decreased [p<0.05]. As exposure to EMF may result in pathological changes then lead to sub fertility and infertility, Since in our study 0.7 g/kg Ocimum basilicum have significantly Preventive effect on uterus cells damages by reducing number of Apoptotic cells, so it seems that using it can be effective for treatment in emf exposed Rat

Compartments of Quercetin and Allium cepa [onion] on blood glucose in diabetic rats

The major concern in diabetes is increased oxidative stress. Maintaining a balance between reactive oxygen species [ROS] and antioxidants is a major mechanism in preventing damage from oxidative stress. Quercetin [QR] is a well-known flavonoid and a strong antioxidant derived from the onion, Allium cepa, and it has been shown to reduce oxidative stress in the long-term treatment of streptozotocin [STZ]-induced diabetes in animals. We aimed to study on beneficial effects of QR and Allium cepa on regulation of blood glucose in diabetic model. Methods: Seventy male 8-week-old Wistar albino rats weighing 250 +/- 10 g were obtained, the rats were divided into seven groups comprising ten animals in each group. Diabetes induced with single intraperitoneal injection of STZ. Diabetic rats treated with quercetin [15mg/kg/day, started 48 hours after STZ injection] and Allium cepa juice [2cc/rat/day, started 48 hours after STZ injection]. After 28 days treatment, analysis on blood glucose and insulin was done. The mean TAC showed a significant increase [p < 0.05] in the QR compared to the control, STZ and STZ + QR groups. Both QR and onion Juice had significant effect in lowering blood glucose [355.3 +/- 104 mg/dl and 353.4 +/- 103 mg/dl respectively] [p<0.05] in STZ induced diabetic animals. Both QR and onion juice had lowering effect in Serum insulin in healthy animals and significant increasing effect on serum insulin level in diabetic animals. Both QR and onion juice had good effect in modifying diabetes related biochemical parameters and they are recommended to be studied more in order to being used in the therapy

[Study on phenolics and antioxidant activity of some selected plant of mazandaran province]

Plants are rich in phenolics [phenolic acids, flavonoids and Tannins]. The interest in phenolics has been increased by recent reports of their antioxidant activities. These antioxidant nutrients from food sources reduce the harmful effects of oxidative stress and offer health advantages. Total phenolics, flavonoids contents and antioxidant capacity of seven culinary plants of Mazandaran province were investigated and compared to Rosemary. Methanolic extracts of dried powdered aerial parts of plants were prepared. Total phenolic compounds in extracts were determined spectrophotometrically using the Folin- Ciocalteu reagent. Antioxidant activity assessed by using 2, 2-diphenyl-1-picrylhydrazyl [DPPH] method. The total phenolic content of plants extracts ranged from 38.27 to 58.45 mgGAEg[1] [Gallic acid equivalent/g dried weight], flavonoid content ranged from 25.5 to 182.23 mgQUEg[1] [mg Quercetin equivalent/g dried weight] and IC[50] ranges from 55.52 to 489.9 microgml[-1]. Among the 7 different plants, Marrubium vulgare contained a higher level of phenolics and high antioxidant capacity compared to Rosemary, while Mentha spicata had the lowest content of phenolics. The antioxidant capacity determined by the 2, 2-diphenyl-1-picrylhydrazyl [DPPH] method was lowest for of Mentha spicata. These results clearly showed the antioxidant activity could be correlated with the phenolic components content in the extracts

[Treatment effects of onion on spermatogenesis in streptozotocin-induced diabetic rat]

Onion has significant blood sugar lowering action. Antioxidants have essential effect on spermatogenesis and sperm parameters. Streptozotocin can dioceses oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Wistar male rat [n=30] were allocated into three groups, control group [n=10] and onion [O] group that received 1cc/rat [gavages]; [n=10], and Diabetic group that received 55mg/kg [IP] streptozotocin [STZ] [n=20] which was subdivided to two groups of 10; STZ group and treatment group. Treatment group received 55 mg/ kg [IP] STZ plus 15 mg/kg [O], daily for, 4 weeks, respectively; however, the control group just received an equal volume of distilled water daily[gavages]. Diabetes was induced by a single [IP] injection of streptozotocin [55mg/kg]. Animals were kept in standard condition. In 28 day after inducing diabetic 5cc blood were collected for testestrone, TAC, MDA and Ox-LDL levels and testes tissues of Rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis. Sperm population, percentage of sperm viability and motility and Serum total testosterones significantly increased in group that has received 1cc/rat onion [p<0.05] in significantly decreased in comparison to control group [p<0.05]. Since in our study 1cc/rat onion have significantly preventive effect on Sperm percentage of viability and motility and serum total testosterones by reducing level of Reactive Oxygen Species [ROS] in serum, so it seems that using it can be effective for sperm healthy parameters in Diabetic Rat

Effects of danae racernosa on spermatogenesis in rat

Danae racemosa is used medicinally and as a culinary spice. Its constituents are stated to have antithrombotic, antihepatotoxic, antinociceptive and antioxidant. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Wistar male rat [n=30] were allocated into three groups, control [n=10] and two test groups [each of ten]. Animals in test groups were subdivided into groups of 2 that received fresh extract equivalent to 200 and 400 mg/Kg/Rat/day Danae racemosa extract. Fresh Danae racemosa extract was administered with gavages for 28 consecutive days. Animals were kept in standard condition. On twenty eighth day, the testes of rats in the all groups were removed and sperm was collected from epididymis and was prepared for analysis. The percentage of sperm viability and motility in both test groups significantly increased [p<0.05], but the sperm concentration significantly increased only in the group that received the high dose of Danae. racemosa extracted, [p<0.05]. It was evident that there was no difference on sperm morphology and testis weight in test groups comparing to control group. In our study Danae racemosa extract has significantly affected the sperm number and percentage of viability and motility; it seems that using 400 mg/Kg/Rat/day of Danae racemosa extract is effective in sperm health parameters

Protective effects of Quercetin on spermatogenesis in streptozotocin-induced diabetic rat

Quercetin is a strong antioxidant and long-term treatment of STZdiabetic animals and it has been shown to reduce oxidative stress. antioxidants have essential effect on spermatogenesis and sperm parameters. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Wistar male rat [n=40] were allocated into three groups, control group [n=10] and Quercetin [QR] group that received 15mg/kg [IP] QR, [n=10], and Diabetic group that received 55 mg/kg [IP] streptozotocin [STZ] [n=20] which was subdivided to two groups of 10; STZ group and treatment group. Treatment group received 55 mg/kg [IP] STZ plus15mg/kg QR, daily for,4 weeks, respectively; however, the control group just received an equal volume of distilled water daily [IP]. Diabetes was induced by a single intra peritonea injection of streptozotocin [55 mg/kg]. Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for testosterone, TAC, MDA and Ox-LDL levels and testes tissues of Rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis. Sperm population, percentage of sperm viability and motility significantly increased in group that has received 15 mg/kg [IP] Quercetin [P < 0.05] in comparison to control and experimental groups. Since in our study 15 mg/kg [IP] Quercetin have significantly Preventive effect on Sperm percentage of viability and motility by reducing level of Reactive Oxygen Species [ROS] in serum, so it seems that using it can be effective for sperm healthy parameters in Diabetic Rat

Evaluation effects of quercetin on liver apoptosis in streptozotocin-induced diabetic rat

Quercetin is a strong antioxidant and long-term treatment of STZdiabetic animals and it has been shown to reduce oxidative stress. Antioxidants have essential effect on spermatogenesis and sperm parameters. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Wistar male rat [n=40] were allocated into three groups, control group [n=10] and Quercetin [QR] group that received 15mg/kg [IP] QR, [n=10], and Diabetic group that received 55mg/kg [IP] streptozotocin [STZ] [n=20] which was subdivided to two groups of 10; STZ group and treatment group. Treatment group received 55mg/kg [IP] STZ plus15mg/kg QR, daily for,4 weeks, respectively; however, the control group just received an equal volume of distilled water daily[IP]. Diabetes was induced by a single [IP] injection of streptozotocin [55mg/kg]. Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for TAC,MDA and Ox-LDL levels and Liver tissues of Rat in whole groups were removed then prepared for Apoptosis analysis by Tunel method. Apoptotic cells significantly decreased in group that has received 15mg/kg [IP] Quercetin [P < 0.05] in comparison to experimental groups [P < 0.05]. Since in our study 15mg/kg [IP] Quercetin have significantly Preventive effect on liver cells damages by reducing number of Apoptotic cells in liver, so it seems that using it can be effective for treatment in Diabetic Rat